crispr design system Search Results


pcr genotyping  (Transnetyx)
99
Transnetyx pcr genotyping
Pcr Genotyping, supplied by Transnetyx, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr genotyping/product/Transnetyx
Average 99 stars, based on 1 article reviews
pcr genotyping - by Bioz Stars, 2026-05
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grna designer tools  (ATUM Bio)
90
ATUM Bio grna designer tools
Grna Designer Tools, supplied by ATUM Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
grna designer tools - by Bioz Stars, 2026-05
90/100 stars
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crispr design tool  (Broad Institute Inc)
90
Broad Institute Inc crispr design tool
Crispr Design Tool, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr design tool/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
crispr design tool - by Bioz Stars, 2026-05
90/100 stars
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gencrispr grna design tool  (GenScript corporation)
90
GenScript corporation gencrispr grna design tool
Gencrispr Grna Design Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gencrispr grna design tool/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gencrispr grna design tool - by Bioz Stars, 2026-05
90/100 stars
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crispr dna design tool  (ATUM Bio)
90
ATUM Bio crispr dna design tool
Crispr Dna Design Tool, supplied by ATUM Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr dna design tool/product/ATUM Bio
Average 90 stars, based on 1 article reviews
crispr dna design tool - by Bioz Stars, 2026-05
90/100 stars
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sanger crispr webtool  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc sanger crispr webtool
Sanger Crispr Webtool, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sanger crispr webtool/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
sanger crispr webtool - by Bioz Stars, 2026-05
90/100 stars
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crispr tool  (GenScript corporation)
90
GenScript corporation crispr tool
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr tool/product/GenScript corporation
Average 90 stars, based on 1 article reviews
crispr tool - by Bioz Stars, 2026-05
90/100 stars
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crispr design assistance  (Vir Biotechnology)
90
Vir Biotechnology crispr design assistance
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr Design Assistance, supplied by Vir Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr design assistance/product/Vir Biotechnology
Average 90 stars, based on 1 article reviews
crispr design assistance - by Bioz Stars, 2026-05
90/100 stars
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crispr-p online primer design software  (PrimerDesign Inc)
90
PrimerDesign Inc crispr-p online primer design software
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr P Online Primer Design Software, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr-p online primer design software/product/PrimerDesign Inc
Average 90 stars, based on 1 article reviews
crispr-p online primer design software - by Bioz Stars, 2026-05
90/100 stars
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crispr design tool  (Blue Heron Biotech)
90
Blue Heron Biotech crispr design tool
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr Design Tool, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr design tool/product/Blue Heron Biotech
Average 90 stars, based on 1 article reviews
crispr design tool - by Bioz Stars, 2026-05
90/100 stars
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crispr guide design tools  (Metabion International AG)
90
Metabion International AG crispr guide design tools
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr Guide Design Tools, supplied by Metabion International AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr guide design tools/product/Metabion International AG
Average 90 stars, based on 1 article reviews
crispr guide design tools - by Bioz Stars, 2026-05
90/100 stars
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crispr sgrna design tool  (GenScript corporation)
90
GenScript corporation crispr sgrna design tool
Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the <t>CRISPR/Cas9</t> and LoxP systems. <t>Two</t> <t>sgRNAs</t> were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Crispr Sgrna Design Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr sgrna design tool/product/GenScript corporation
Average 90 stars, based on 1 article reviews
crispr sgrna design tool - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the CRISPR/Cas9 and LoxP systems. Two sgRNAs were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.

Journal: Viruses

Article Title: Genomic Characterization and gE/gI-Deleted Strain Construction of Novel PRV Variants Isolated in Central China

doi: 10.3390/v15061237

Figure Lengend Snippet: Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the CRISPR/Cas9 and LoxP systems. Two sgRNAs were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.

Article Snippet: sgRNAs targeting the gE and gI genes were designed using an online CRISPR tool ( https://www.genscript.com/gRNA-design-tool.html , accessed on 15 May 2021).

Techniques: Mutagenesis, Virus, CRISPR